Myeloid Plasticity of Neutrophil Precursors Contributes to Eosinophil Emergency Hematopoiesis

Abstract Eosinophils and neutrophils are heterogenous cells that play diverse roles in homeostasis inflammation. Tissue eosinophil origins poorly understood, posing challenges the functional interpretation of heterogeneous phenotypes. New evidence from our lab shows unexpected hematopoietic plasticity Ly6G(+)IL-5Rα(+) band-stage with potential to contribute lineage. Using flow cytometry multi-omics approaches mouse human tissue samples derived healthy allergic conditions, we tested hypothesis neutrophil contributes diversity We found at least two distinct populations a baseline, one which expressed classical markers (S100A8/9) while other both (MPO, ELANE) eosinophil-specific (EPX, PRG2) granule proteins, indicating an intermediate state. In treated IL-5/G-CSF vitro, subset mixed characteristics acquired granular proteins cell surface (Siglec8, CCR3). asthma model, using scRNA-seq, overlap eosinophils remodeling functions. also identified activated playing unique Our findings implicate emergency hematopoiesis driven by as overlooked process driving persistence Supported grants GSK ISS NIH R01